Nuclear receptors (NRs) are vital proteins that regulate key physiological functions in the body. These proteins play a crucial role in sensing thyroid hormones, steroids, and various lipophilic molecules. With 48 genes in the human genome coding for NRs, this family represents the largest group of eukaryotic transcription factors. They are involved in an array of processes from development to metabolism, making them significant targets for therapeutic interventions.
At Creative Bioarray, we provide an extensive suite of cell-based nuclear receptor (NR) assays designed to evaluate the interactions of drug candidates with these critical proteins. Our assays are crafted to measure the effects of potential therapeutic compounds on nuclear receptor activity, which can reveal important insights into their selectivity and potential off-target interactions.
Overview of Our Services
Comprehensive Assay Panel
We offer a robust and optimized panel of functional nuclear receptor assays that assess steroid cofactor recruitment to the receptors being tested. Our collection includes over 20 different cellular assays, measuring activation through pathways such as nuclear translocation and co-activator interaction.
Flexible Screening Solutions
Our services provide a personalized approach to nuclear receptor screening. Clients can select from a library of 31 NR assays to create tailored assay panels that address specific research needs or therapeutic targets.
Quantitative Affinity Measurements
We conduct quantitative studies of compound-NR interactions using 10-point dose-response curves executed under optimized conditions. This allows for detailed analysis of binding affinities across any NR/compound combination.
Multiple Functional Readouts
Our platform enables researchers to study various pharmacological effects of compounds on the same nuclear receptor, utilizing diverse readouts including protein interaction studies and translocation analytics.
Key Advantages
- Extensive Portfolio: Access the largest collection of optimized, cell-based nuclear receptor assays with reproducible results.
- Agonist and Antagonist Capabilities: Analyze both partial and full agonist and antagonist activities.
- Customization Options: Choose from predefined panels or create your own, allowing flexibility in experimental design.
- Rapid Turnaround: Enjoy quick processing times with comprehensive reports evaluating the efficacy and selectivity of your compounds.
